However, total root volume decreased significantly under the

To investigate whether particular domains within MED16 might be responsible for the activation of specific genes under different stresses, complementation experiments were used to test the ability of three different truncated MED16 versions to restore cold, dark and UV inducible expression. Some truncated versions were able to complement the mutant but the degree of complementation varied amongst transgenic lines. Experiments were conducted to study the function of KIN10, an interacting protein of MED16 that appears to play a role similar to MED16 in regulation of stress genes and tolerance.

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Photosynthesis, stomatal conductance and transpiration were also measured to examine the shoot behaviour under the same environmental conditions. The results showed that total root volume (after 14 days) under the combined effect of elevated CO2 and temperature and mean root diameter for all experiments increased significantly with increasing water stress. However, total root volume decreased significantly under the effect of water stress independently and in combination with elevated CO2 and temperature.

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